Year-end summary: problems that should be noted when ELISA operation

The outer diameter of the probe domestic P156-G flat nickel plated needle is 2.36mm.

The enzyme-linked immunosorbent assay kit (http://) is a commonly used drug residue detection method, which is fast, simple, accurate and sensitive compared with instrumental analysis technology, but The requirements for experimental operating conditions are relatively strict. The experimental conditions are mainly composed of the operating environment of the laboratory and the operational proficiency of the operators. However, the requirements of the elisa kit for the laboratory operating environment are relatively low among several drug residue detection methods. Since most of the test procedures are performed by the experimenter, the human error is relatively serious. I combined the work experience of several years to introduce the problems that should be paid attention to when performing ELISA.
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$Width="512" height="143" src="http://i.bosscdn.com/blog/20/17/10/21165637710279.jpg" />There is a solution to the problem when using the kit The result of the test has no absorbance value or the absorbance value is very low.The reason may be caused by the expiration date of the kit, the contents of the kit used are not the same batch, and no enzyme label or reagent is added. The contents of the cartridge are not sufficiently warmed, the incubation temperature is too low, etc., and the corresponding solution is to replace the kits within the expiration date with the contents of the same batch of kits, according to the contents of the kit. Follow the steps in the kit instructions, and return the contents of the kit to the temperature. Then carefully observe the temperature of the incubator during the whole process of the experiment. Elisa kit negative control and positive control analysis Elisa kit negative control and positive control: If the positive and negative controls have correct results, the results of the kit operation can be roughly judged. blank control: For the ELISA reagent, the blank control is actually without the specimen and no enzyme is used as a control. The blank control primarily measures the absorbance of the system. Blank controls are especially important when measuring single wavelengths. Our company represents thousands of ELISA kits, tens of thousands of antibodies, and represents nearly 10,000 world-class products, involving molecular biology, molecular immunology, and cell biology. And other fields of life science research. In order to save your time, we can also provide generation testing services. </p> </div> </div> <div class="tech-detail-share">  <div class="bdsharebuttonbox"> <a href="#" class ="bds_qzone" data-cmd="qzone" title="Share to QQ space"></a> <a href="#" class="bds_tsina" data-cmd="tsina" title="Share to Sina Wei Bo"></a> <a href="#" class="bds_weixin" data-cmd="weixin" title="Share to WeChat"></a> <span>Share to:</span> </ Div> <script>window._bd_share_config = { "common": { "bdSnsKey": {}, "bdText": "", "bdMini": "1", "bdMiniList": false, "bdPic": "", "bdStyle": "2", "bdSize": "16" }, "share": {} }; with (document) 0[(getElementsByTagName($

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Year-end summary: problems that should be noted when ELISA operation - Database & Sql Blog Articles

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